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Researcher 4- Division of Surgical Oncology

Application Procedure:

To apply for this job, click here. Please search by Job Opening ID 339351.

Job Duties

The UMN has suspended hiring for most positions. However, we will continue hiring for positions critical to the University's mission and operations at this time. Applications are being accepted for this position and you are encouraged to apply. In-person interviews are suspended indefinitely and will be replaced by interviews in a virtual format. Thank you for your continued interest in working at the University of Minnesota.

Specific Duties:

Independent Conduct of Scientific Experiments (80%)
•    Maintenance and treatment of cells in culture as needed.
•    Care of KPC genetic or related mice in facilities.
•    Establishment of genetic tumor mouse models, treatment of animals, and assessment of treatment response via viability assays, immune assays, and cytokine profiling.
•    Perform mouse necropsy to analyze mouse tissues for immune cell populations, phenotypes, and underlying mechanisms.
•    Processing and gradient centrifugation of peripheral blood samples.
•    Processing and dissociation of fresh tissue specimens.
•    Preparation and processing of single cell suspensions for multi-color flow cytometry.
•    Preparation and processing of fresh tissue for immunofluorescence or histocytometry.
•    Preparation and processing of single cell suspensions or fresh tissue for genetic analysis including bulk RNAseq, single cell RNAseq, and possible exome sequencing or PCR.
•    Perform routine molecular biology analyses as needed.
•    Evaluate the effects of therapeutics on cells, tissue samples, and in animal models as above.
•    Develop and troubleshoot new techniques and assays.
•    Develop new protocols and ensure they are followed precisely.
•    Independently problem solve/resolve issues with scientific experiments and recommend solutions.

Laboratory Management (10%)
•    Maintain laboratory safety by ensuring that the lab is compliant with appropriate safety regulations and that lab specific protocols are established and followed.
•    Ensure compliance with record keeping and safety training.
•    Oversee the tracking and restocking of lab supplies.
•    Perform validation exercises for all aspects of reagent and supply preparation and processing.
•    Other maintenance and supply duties as required.

Data Management (5%)
•    Analyze, summarize, and report experimental data to Principal Investigator as well as other members of the research team as needed.
•    Oversee meticulous record keeping of all experiments and projects.
•    Other data management duties as required.

Literature review, R&D, and additional duties (5%) 
•    Review relevant research literature to facilitate job performance.
•    Attendance at weekly lab meetings.
•    Collaborate on the preparation of protocols, presentations, and manuscripts.
•    Oversee ongoing R&D operations as time allows and as assigned by Principal Investigator.
•    Other duties as required.



Job Qualifications

All required qualifications must be documented on application materials.

Required Qualifications:
•    Masters degree and 3 years of related experience or BA/BS and at least 4 years of experience working in a biological lab. 
•    Experience in immunology, molecular biology, and cell biology or related field.
•    Ability to work independently in a rapidly changing scientific environment and conduct experiments independently.
•    Strong written and verbal communication skills.
•    Analytical skills to process complex and varied scientific problems and identify solutions.
•    Demonstrated attention to detail and ability to follow directions/protocols. 
•    Meticulous organizational and time management skills.
•    Experience with Microsoft Excel and PowerPoint or similar programs for data management and presentation creation.
•    Must be able to stand at a laboratory bench or hood for extended periods of time and lift up to 40 lbs.
•    Must be able to work flexible hours as needed by project.
•    Must be able to work on-site in a laboratory setting


Preferred Qualifications:
• Graduate-level coursework.
•    Previous experience working in a BSL2 laboratory setting.
•    Prior experience with blood processing, centrifugation, and tissue dissociation.
•    Prior experience with multi-color advanced flow cytometry.
•    Prior experience with single-cell RNAseq.
•    Prior experience with immune cell culture and basic immunoassays.
•    Prior experience with tissue immunohistochemistry or histocytometry.
•    Prior experience with animal studies and care using mice.
•    Experience using the following equipment is preferred: fluorometer, spectrophotometer, spectrometer, centrifuge, speed-vac, microscopes, biological safety cabinet, autoclave, incubators, manual pipet, electronic pipet, digital balance, centrifuges, lab chillers and water baths, fume hoods, gas cylinders, pH meter, sonicator, flow cytometer, 7300 Real-Time PCR System, etc.

Institution Description

The University of Minnesota, Twin Cities (UMTC), is among the largest public research universities in the country, offering undergraduate, graduate, and professional students a multitude of opportunities for study and research.  Located at the heart of one of the nation's most vibrant, diverse metropolitan communities, students on the campuses in Minneapolis and St. Paul benefit from extensive partnerships with world-renowned health centers, international corporations, government agencies, and arts, nonprofit, and public service organizations.

To learn more about the Department of Surgery please go to https://med.umn.edu/surgery.

Benefits

The University of Minnesota offers a comprehensive benefits package including:

  • Competitive wages, paid holidays, vacation and sick leave
  •  Low cost medical, dental, and pharmacy plans
  • Health care and dependent daycare flexible spending accounts
  • Excellent retirement plans with employer match
  • Disability and employer-paid life insurance
  • Wellbeing program with reduced insurance premiums
  • Tuition reimbursement opportunities covering 75%-100% of eligible tuition
  • Student loan forgiveness opportunity
  • Opportunities for growth and promotion
  • Employee Assistance Program

For more information regarding benefits: here

Application Due Date: 2021-04-26
Job Start Date: 2021-05-03
Salary: Dependent on qualifications and experience.
City: Minneapolis
State/Province/Region: MN
Institution: Department of Surgery - University of Minnesota
Reports To: Jacob Ankeny
Staff # Reporting to Position: 0
Reports In Office: Department of Surgery

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